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KMID : 0606920110190020211
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Biomolecules & Therapeutics
2011 Volume.19 No. 2 p.211 ~ p.217
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A Fermented Ginseng Extract, BST204, Inhibits Proliferation and Motility of Human Colon Cancer Cells
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Park Jong-Won
Lee Jae-Cheol
Ann So-Ra
Seo Dong-Wan
Choi Wahn-Soo
Yoo Young-Hyo
Park Sun-Kyu
Choi Jung-Young
Um Sung-Hee
Ahn Seong-Hoon
Han Jeung-Whan
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Abstract
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Panax ginseng CA Meyer, a herb from the Araliaceae, has traditionally been used as a medicinal plant in Asian countries. Ginseng extract fermented by ginsenoside-β-glucosidase treatment is enriched in ginsenosides such as Rh2 and Rg3. Here we show that a fermented ginseng extract, BST204, has anti-proliferative and anti-invasive effects on HT-29 human colon cancer cells. Treatment of HT-29 cells with BST204 induced cell cycle arrest at G1 phase without progression to apoptosis. This cell cycle arrest was accompanied by up-regulation of tumor suppressor proteins, p53 and p21WAF1/Cip1, down-regulation of the cyclin-dependent kinase/cyclins, Cdk2, cyclin E, and cyclin D1 involved in G1 or G1/S transition, and decrease in the phosphorylated form of retinoblastoma protein. In addition, BST204 suppressed the migration of HT-29 cells induced by 12-O-tetradecanoylphorbol-13-acetate, which correlated with the inhibition of metalloproteinase-9 activity and extracellular signal-regulated kinase activity. The effects of BST204 on the proliferation and the invasiveness of HT-29 cells were similar to those of Rh2. Taken together, the results suggest that fermentation of ginseng extract with ginsenoside-β-glucosidase enhanced the anti-proliferative and the anti-invasive activity against human colon cancer cells and these anti-tumor effects of BST204 might be mediated in part by enriched Rh2.
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KEYWORD
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BST204, Ginsenoside, Cell cycle, Cell migration, Cell proliferation, Colon cancer
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